Serum Protein Electrophoresis (SPEP) is a laboratory test that separates proteins in the blood based on their size and charge. It is primarily used to diagnose plasma cell disorders like multiple myeloma, amyloidosis, and chronic inflammatory conditions by measuring the different types of proteins in the serum.
Indications for Serum Protein Electrophoresis Test:
● Suspected plasma cell disorders: SPEP is often used when there is suspicion of conditions like multiple myeloma, Waldenström macroglobulinemia, or light chain amyloidosis.
● Chronic inflammation: It helps in diagnosing inflammatory conditions such as rheumatoid arthritis and systemic lupus erythematosus (SLE).
● Nephrotic syndrome: To evaluate the protein levels in the blood and urine for patients with proteinuria or kidney dysfunction.
How Serum Protein Electrophoresis Works:
● A blood sample is taken and serum proteins are separated using an electrophoresis technique, where proteins move in an electric field according to their size and charge. The separated proteins are then analyzed to detect any abnormal spikes or patterns.
Normal Range:
● Albumin: 54-65% of total protein.
● Alpha-1 globulin: 1-3%.
● Alpha-2 globulin: 6-10%.
● Beta globulin: 7-12%.
● Gamma globulin: 10-20%.
● Monoclonal spike: Normal results should not show an abnormal spike (or “M spike”) in the gamma region, which is seen in plasma cell disorders like multiple myeloma.
Abnormal Test Significance:
● Monoclonal gammopathy: An abnormal M spike suggests the presence of multiple myeloma, amyloidosis, or monoclonal gammopathy of undetermined significance (MGUS).
● Decreased albumin levels: Can indicate liver disease, kidney disease, or malnutrition.
● Increased alpha-2 globulins: May indicate inflammation or nephrotic syndrome.
Management Based on Results:
● For monoclonal gammopathy: Further tests, such as bone marrow biopsy or immunofixation electrophoresis, may be necessary to confirm conditions like multiple myeloma or amyloidosis.
● For chronic inflammation: Treating the underlying condition may help normalize protein levels.
Price in India:
Approx. ₹2,000 – ₹4,000 for serum protein electrophoresis.
Frequently Asked Questions
How is serum protein electrophoresis different from other tests?
It measures the levels and types of proteins in the blood, specifically detecting abnormal protein production that occurs in conditions like multiple myeloma.
Can SPEP detect cancer?
Yes, it can help in diagnosing cancers like multiple myeloma, which is characterized by abnormal plasma cell production.
Disclaimer: Serum protein electrophoresis is part of a broader diagnostic workup for plasma cell disorders and should be interpreted alongside clinical findings and other tests.
How HealthPil Can Help:
At HealthPil, we offer access to hematologists who can guide you through the interpretation of your SPEP results and provide the necessary next steps for treatment and diagnosis of underlying conditions.
